Effects of an Antibody to a Highly Purified Na ⁺ , K ⁺ -ATPase from Canine Renal Medulla: Separation of the “Holoenzyme Antibody” into Catalytic and Cardiac Glycoside Receptor-Specific Components

An antiserum was prepared against a highly purified Na(+), K(+)-adenosine triphosphatase (ATP phosphohydrolase, EC 3.6.1.3). Purification and fractionation yielded two globulin components, one of which appears specific for a digitalis glycoside receptor site or binding conformation and the other for a catalytic site or conformation.