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October 1, 1992Journal of Biological ChemistryOpen Access

Cell-specific translation of S-adenosylmethionine decarboxylase mRNA. Regulation by the 5' transcript leader.

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Population

T lymphocyte origin cells (Jurkat), fibroblasts, and adrenal carcinoma line (Y1)

Comparison

Chimeric expression construct replacing the… vs Endogenous AdoMetDC mRNA, or unmutated constructs

Design

Preclinical

Authors

JHJohn R. HillGeorge Washington UniversityDMDavid R. MorrisAberystwyth University

Discussion

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Implication

No immediate clinical implications from this animal study; leaves open uORF targeting in human T-cell disorders.

Structured PICO

P
Population
T lymphocyte origin cells (Jurkat), fibroblasts, and adrenal carcinoma line (Y1)
I
Intervention
Chimeric expression construct replacing the 5'-TL of human growth hormone gene with AdoMetDC 5'-TL, and mutations removing the uORF
C
Comparator
Endogenous AdoMetDC mRNA, or unmutated constructs
O
Outcome
Polysomal distribution of mRNAsurrogate

The uORF in the AdoMetDC 5'-transcript leader acts as a negative element modulating cell-specific polysomal distribution of its mRNA.

Cite This Study

Hill et al. (1992) studied this question.

synapsesocial.com/papers/6a83d1813fd01c19a6c580fehttps://doi.org/10.1016/s0021-9258(19)36695-5
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