What question did this study set out to answer?

Characterize a newly isolated genomic clone of the mouse S-adenosylmethionine decarboxylase (AdoMetDC) gene and evaluate its structure, evolutionary origin, and catalytic activity.

March 1, 1995Journal of Biological ChemistryOpen Access

Cloning and Sequencing of an Intronless Mouse S-Adenosylmethionine Decarboxylase Gene Coding for a Functional Enzyme Strongly Expressed in the Liver

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Authors

KPKent PerssonInstitution of Structural Engineers IHIngvar HolmMalmö University OHOlle HebyUniversity of Helsinki

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Implication

Molecular analysis identifies an active retroposed S-adenosylmethionine decarboxylase gene in mice, demonstrating functional retrogene expression predominantly in liver tissue.

Key Points

Characterize a newly isolated genomic clone of the mouse S-adenosylmethionine decarboxylase (AdoMetDC) gene and evaluate its structure, evolutionary origin, and catalytic activity.
Isolated genomic clones from a mouse cosmid library and sequenced the 1002-nucleotide open reading frame.
Confirmed genomic presence via PCR and evaluated tissue-specific expression using primer extension analysis across various mouse cell lines and organs.
Expressed the coding sequence in an AdoMetDC-deficient Escherichia coli strain (HT551) to assess proenzyme processing and catalytic decarboxylation activity.
Sequencing revealed an intronless AdoMetDC gene with 3 base substitutions relative to cDNA, flanked by 13-base pair direct repeats and a 3' poly(dA) tract characteristic of retroposition.
Primer extension confirmed active transcript expression across diverse mouse tissues, with the highest expression levels detected in the liver.
Recombinant expression produced a 38-kDa proenzyme that cleaved into functional 31-kDa alpha and 8-kDa beta subunits, exhibiting specific decarboxylation activity comparable to purified recombinant human AdoMetDC.

Cite This Study

Persson et al. (1995) studied this question.

synapsesocial.com/papers/6a8a3cf24de818ad79d4500d https://doi.org/10.1074/jbc.270.10.5642

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Implication

Molecular analysis identifies an active retroposed S-adenosylmethionine decarboxylase gene in mice, demonstrating functional retrogene expression predominantly in liver tissue.

Cloning and Sequencing of an Intronless Mouse S-Adenosylmethionine Decarboxylase Gene Coding for a Functional Enzyme Strongly Expressed in the Liver

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Implication

Key Points

Cite This Study

Cloning and Sequencing of an Intronless Mouse S-Adenosylmethionine Decarboxylase Gene Coding for a Functional Enzyme Strongly Expressed in the Liver

Discussion

Member takes

Implication

Key Points

Cite This Study

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