Membrane-Associated DNA in the Cytoplasm of Diploid Human Lymphocytes
Authors
Discussion
Loading...
Member takes
Implication
In vitro study identifies membrane-associated DNA in human diploid lymphocytes, revealing distinct synthesis timing and physical properties.
Key Points
- Characterize a novel species of DNA associated with the plasma membrane in continuously growing diploid human lymphocytes.
- Analyzed continuously growing cultures of human diploid lymphocytes to detect non-nuclear cytoplasmic DNA.
- Compared the subcellular localization, physical properties, and cell-cycle timing of synthesis of the isolated DNA against nuclear and mitochondrial DNA.
- Detected a distinct species of DNA physically associated with the lymphocyte plasma membrane.
- Demonstrated that this membrane-associated DNA differs substantially from nuclear and mitochondrial DNA in its cellular location, physical traits, and replication schedule across the cell cycle.
Cite This Study
Lerner et al. (1971) studied this question.