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A method is described for blood plasma volatile fatty acids using direct steam distillation with an anti-foaming agent. Free fatty acids are collected on a microcondenser prior to gas liquid chromatographic analysis. The method is accurate through a wide range for acetate (.05 to 2.50 μ moles per liter), propionate, isobutyrate, butyrate, and valerate (5.0 to 220.0 μ moles per liter). However, with increasing concentration there is decreasing precision. The low precision at high concentrations is not serious, since precision is good within the physiologic range. Volatile fatty acids from aqueous solutions and those added to sheep and cattle blood plasma were quantitatively recovered. A comparison was made of the volatile fatty acid in the jugular blood of sheep under conditions of fasting and refeeding.
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Ross et al. (1971) studied this question.
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