What question did this study set out to answer?

To establish a purification procedure for streptococcal M protein and characterize the physical, chemical, and serological properties of its isolated fragments.

September 1, 1968The Journal of ImmunologyOpen Access

Purification of Two Fragments of M Protein from a Strain of Group A, Type 12 Streptococcus

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Authors

RJRudolph H. JohnsonUnited States Department of Veterans Affairs KVKenneth L. VostiStanford Medicine

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Implication

Biochemical analysis demonstrates two homogeneous M protein fragments in group A streptococcus, suggesting the protein consists of repeating structural units.

Key Points

To establish a purification procedure for streptococcal M protein and characterize the physical, chemical, and serological properties of its isolated fragments.
Extracted and purified M protein from a strain of group A, type 12 hemolytic streptococcus into two distinct fractions designated 'a' and 'b'.
Evaluated homogeneity, mobility, and purity using polyacrylamide gel electrophoresis, analytic ultracentrifugation, sucrose density gradients, and chemical assays for nucleic acids and methylpentoses.
Characterized molecular structure and antigenic activity using two-dimensional peptide mapping, type-specific serological testing, and indirect hemagglutination.
Isolated two homogeneous fractions ('a' and 'b') free of nucleic acids and methylpentoses that retained all original serological activity.
Demonstrated similar two-dimensional peptide maps between both fractions despite different migration profiles on polyacrylamide gels and analytic ultracentrifugation.
Identified both type-specific M determinants and a shared nontype-specific antigenic determinant on both fragments via indirect hemagglutination.

Cite This Study

Johnson et al. (1968) studied this question.

synapsesocial.com/papers/6a83fbf5448cdc0007ee8ce9 https://doi.org/10.4049/jimmunol.101.3.381

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Implication

Biochemical analysis demonstrates two homogeneous M protein fragments in group A streptococcus, suggesting the protein consists of repeating structural units.

Purification of Two Fragments of M Protein from a Strain of Group A, Type 12 Streptococcus

Discussion

Member takes

Implication

Key Points

Cite This Study

Purification of Two Fragments of M Protein from a Strain of Group A, Type 12 Streptococcus

Discussion

Member takes

Implication

Key Points

Cite This Study

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