What question did this study set out to answer?

To determine whether endothelial dysfunction develops after brief, moderate low-flow myocardial ischemia and subsequent reperfusion in an in vivo porcine model.

December 1, 1990CirculationOpen Access

Effects of brief coronary occlusion and reperfusion on porcine coronary artery reactivity.

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Why the study?

Does brief coronary occlusion and reperfusion impair endothelial function in porcine coronary arteries?

Population

Open-chest swine

Comparison

15 minutes of critical, subtotal left anterior… vs Normally perfused left circumflex coronary…

Design

Preclinical

Follow-up

60 minutes of reperfusion

Authors

JHJohn P. HeadrickGriffith University DADebra A. AngelloUniversity of Virginia RBRobert M. BerneSt George's Hospital

Discussion

Member takes

Implication

Indicates ischemia-reperfusion induces coronary endothelial dysfunction in this model; leaves open translation to human coronary disease.

Key Points

To determine whether endothelial dysfunction develops after brief, moderate low-flow myocardial ischemia and subsequent reperfusion in an in vivo porcine model.
Open-chest swine were subjected to 15 minutes of 80% subtotal left anterior descending (LAD) coronary artery occlusion followed by 60 minutes of reperfusion.
Regional coronary blood flow was assessed using the radiolabeled microsphere technique during occlusion and reperfusion.
Vascular reactivity of isolated LAD rings was compared in vitro against normally perfused left circumflex coronary artery control rings using endothelium-dependent (ADP, bradykinin) and endothelium-independent (sodium nitroprusside, adenosine) dilators, as well as serotonin.
Endocardial and transmural blood flows recovered fully to preocclusion baseline levels within 60 minutes of reperfusion.
Endothelium-dependent relaxations to adenosine diphosphate and bradykinin were significantly depressed in reperfused LAD rings compared to control circumflex rings (p < 0.05).
Serotonin-induced contractions were significantly greater in occluded-reperfused LAD arteries than in control circumflex arteries (p < 0.05), while relaxations to adenosine and sodium nitroprusside showed no significant difference.

Structured PICO

Does brief coronary occlusion and reperfusion impair endothelial function in porcine coronary arteries?

Population

Open-chest swine

Intervention

15 minutes of critical, subtotal left anterior descending coronary artery occlusion (80%) followed by 60 minutes of reperfusion

Comparator

Normally perfused left circumflex coronary artery (control rings) from the same animals

Outcome

Vascular responses in isolated coronary artery rings (relaxations to ADP, bradykinin, sodium nitroprusside, adenosine, and contractions to serotonin)surrogate

Brief periods of moderate low-flow ischemia followed by reperfusion induce significant endothelial dysfunction and increased contractile sensitivity in porcine coronary arteries.

Cite This Study

Headrick et al. (1990) studied this question.

synapsesocial.com/papers/6a84003db319f6e7ebb28f0d https://doi.org/10.1161/01.cir.82.6.2163

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Effects of brief coronary occlusion and reperfusion on porcine coronary artery reactivity.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effects of brief coronary occlusion and reperfusion on porcine coronary artery reactivity.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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