January 1, 2005Thrombosis and Haemostasis

Recombinant human activated protein C upregulates cyclooxygenase-2 expression in endothelial cells via binding to endothelial cell protein C receptor and activation of protease-activated receptor-1

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Why the study?

Does recombinant human activated protein C upregulate COX-2 expression and prostacyclin release in human umbilical vein endothelial cells?

Population

Human umbilical vein endothelial cells (HUVEC)

Comparison

Recombinant human activated protein C at… vs Zymogen protein C in equimolar concentrations or…

Design

Preclinical

Follow-up

3-9 hours (in vitro)

Authors

SHSarah R. HornBoston University SLSiegfried LangGoethe University Frankfurt KFKenji FukudomeSaga University

Discussion

Member takes

Implication

Suggests a mechanism for rhAPC endothelial effects; leaves open clinical translation pending human studies.

Structured PICO

Does recombinant human activated protein C upregulate COX-2 expression and prostacyclin release in human umbilical vein endothelial cells?

Population

Human umbilical vein endothelial cells (HUVEC)

Intervention

Recombinant human activated protein C (rhAPC) at supra-therapeutical concentrations (500 ng/ml-20 microg/ml)

Comparator

Zymogen protein C in equimolar concentrations or unstimulated control

Outcome

COX-2-mRNA-expression and PGI(2) release (measured as 6-keto PGF(1 alpha))surrogate

rhAPC upregulates COX-2 expression and prostacyclin production in human endothelial cells via EPCR and PAR-1, providing a potential molecular mechanism for its microcirculatory benefits.

Cite This Study

Horn et al. (2005) studied this question.

synapsesocial.com/papers/6a8404285c5b0dd3e07be9fa https://doi.org/10.1160/th04-08-0511

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Recombinant human activated protein C upregulates cyclooxygenase-2 expression in endothelial cells via binding to endothelial cell protein C receptor and activation of protease-activated receptor-1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Recombinant human activated protein C upregulates cyclooxygenase-2 expression in endothelial cells via binding to endothelial cell protein C receptor and activation of protease-activated receptor-1

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider