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A procedure is described for the extraction of lipids from immature soybeans that eliminates artifact formation and provides complete recovery of the lipid, including highly polar glycolipids free of nonlipid substances. The method involves pretreatment and extraction of the beans with hot dilute (0.25%) acetic acid, followed by chloroform‐methanol extraction. Pretreatment and extraction of the tissue with hot dilute acetic acid destroys hydrolytic enzymes and removes organic‐soluble, nonlipid substances that contaminate extracts obtained by chloroform‐methanol extraction. Application of the method to immature soybeans confirmed that phosphatidic acid was largely an artifact of freezing and thawing of the beans, and phosphatidylmethanol was produced via transphosphatidylation of phosphatidylcholine and phosphatidylethanolamine upon extraction with chloroform‐methanol.
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Phillips et al. (1979) studied this question.
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