What question did this study set out to answer?

To immobilize organophosphorus hydrolase using layer-by-layer polyelectrolyte self-assembly and detect the toxic organophosphorus compound paraoxon.

January 29, 2003Journal of the American Chemical Society

Layer-by-Layer Self-Assembled Chitosan/Poly(thiophene-3-acetic acid) and Organophosphorus Hydrolase Multilayers

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Authors

CCCeleste A. ConstantineUniversity of Miami SMSarita V. MelloColgate-Palmolive (United States)ADAnnie DupontUniversity of Miami

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Implication

In vitro study demonstrates stable organophosphorus hydrolase immobilization on polyelectrolyte multilayers for paraoxon detection, highlighting potential for rapid biosensor development.

Key Points

To immobilize organophosphorus hydrolase using layer-by-layer polyelectrolyte self-assembly and detect the toxic organophosphorus compound paraoxon.
Alternated electrostatic adsorption of cationic chitosan and anionic poly(thiophene-3-acetic acid) onto hydrophilic quartz slides using pH-adjusted aqueous solutions.
Monitored multilayer film growth across five bilayers using fluorescence spectroscopy before adsorbing organophosphorus hydrolase onto the construct.
The five-bilayer chitosan/poly(thiophene-3-acetic acid) cushion preserved the structural integrity and enzymatic stability of organophosphorus hydrolase superior to direct solid-substrate adsorption.
Immobilized organophosphorus hydrolase maintained functional catalytic activity by successfully reacting with paraoxon solutions for optical detection.

Cite This Study

Constantine et al. (2003) studied this question.

synapsesocial.com/papers/6a8408ec25efb1e71e83d576 https://doi.org/10.1021/ja028691h

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Implication

In vitro study demonstrates stable organophosphorus hydrolase immobilization on polyelectrolyte multilayers for paraoxon detection, highlighting potential for rapid biosensor development.

Layer-by-Layer Self-Assembled Chitosan/Poly(thiophene-3-acetic acid) and Organophosphorus Hydrolase Multilayers

Discussion

Member takes

Implication

Key Points

Cite This Study

Layer-by-Layer Self-Assembled Chitosan/Poly(thiophene-3-acetic acid) and Organophosphorus Hydrolase Multilayers

Discussion

Member takes

Implication

Key Points

Cite This Study

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