What question did this study set out to answer?

To examine whether insulin-stimulated phosphorylation of the insulin receptor in rat adipocytes depends on divalent cations and cellular energy.

August 15, 1983Biochemical JournalOpen Access

Calcium-dependence of insulin receptor phosphorylation

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Authors

WPWalter E. PlehweEpworth Hospital PWPaul F. WilliamsLawrence Berkeley National Laboratory ICIan D. CatersonThe University of Sydney

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Implication

In vitro study demonstrates calcium- and energy-dependent insulin receptor phosphorylation in rat adipocytes, suggesting calmodulin modulation in early insulin signaling.

Key Points

To examine whether insulin-stimulated phosphorylation of the insulin receptor in rat adipocytes depends on divalent cations and cellular energy.
Isolated rat adipocytes were exposed to insulin for 15 minutes and labeled with 32P in the presence or absence of physiological Ca2+ and Mg2+.
Adipocyte membrane proteins were isolated via immunoprecipitation and characterized using SDS-polyacrylamide gel electrophoresis and autoradiography in the presence of metabolic uncoupler 2,4-dinitrophenol or calmodulin antagonist trifluoperazine.
Insulin stimulated 32P incorporation predominantly into the 95,000 Mr beta-subunit of the insulin receptor, an effect dependent on physiological concentrations of Ca2+ and Mg2+.
Phosphorylation was inhibited by 2,4-dinitrophenol and trifluoperazine, indicating an energy-dependent process that is calcium-dependent, potentially modulated by calmodulin, and independent of glucose transport.

Cite This Study

Plehwe et al. (1983) studied this question.

synapsesocial.com/papers/6a8416ea84161f555bc51fc3 https://doi.org/10.1042/bj2140361

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Implication

In vitro study demonstrates calcium- and energy-dependent insulin receptor phosphorylation in rat adipocytes, suggesting calmodulin modulation in early insulin signaling.

Calcium-dependence of insulin receptor phosphorylation

Discussion

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Implication

Key Points

Cite This Study

Calcium-dependence of insulin receptor phosphorylation

Discussion

Member takes

Implication

Key Points

Cite This Study

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