What question did this study set out to answer?

To determine the ultrastructural localization and extrinsic origins of the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) within the lateral parabrachial region of adult male rats.

September 1, 1986Journal of NeuroscienceOpen Access

Tyrosine hydroxylase in the rat parabrachial region: ultrastructural localization and extrinsic sources of immunoreactivity

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Authors

TMTA MilnerCornell University TJTH JohCornell University VPVM PickelNewYork–Presbyterian Hospital

Discussion

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Implication

Neuroanatomical study uncovers synaptic targets and extrinsic catecholaminergic projections of tyrosine hydroxylase in rat parabrachial regions, suggesting intricate sensory processing networks.

Key Points

To determine the ultrastructural localization and extrinsic origins of the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) within the lateral parabrachial region of adult male rats.
Examined acrolein-fixed coronal brain sections from adult male rats using electron microscopy and TH immunocytochemistry via the peroxidase-antiperoxidase method.
Combined TH immunolabeling with retrograde axonal tracing by unilaterally injecting wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) into the parabrachial region.
TH-immunoreactive perikarya and dendrites constituted only 17% of labeled profiles, mostly received noncatecholaminergic inputs, and localized primarily to the central lateral nucleus.
Labeled axons and axon terminals accounted for the remaining 83% of profiles, dividing into small Type I (0.3–0.6 µm) and large dense-core vesicle-containing Type II (0.8–1.4 µm) terminals morphologically resembling dopaminergic inputs.
Retrograde tracing confirmed that multiple extrinsic catecholaminergic cell groups project directly to the lateral parabrachial region, evidenced by dense WGA-HRP reaction product within TH-positive neurons.

Cite This Study

Milner et al. (1986) studied this question.

synapsesocial.com/papers/6a842716064c65267cc6bcbf https://doi.org/10.1523/jneurosci.06-09-02585.1986

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September 1, 1986Journal of NeuroscienceOpen Access

Tyrosine hydroxylase in the rat parabrachial region: ultrastructural localization and extrinsic sources of immunoreactivity

View Full Paper

Ask AI

Bookmark

Authors

TMTA MilnerCornell University TJTH JohCornell University VPVM PickelNewYork–Presbyterian Hospital

Discussion

Member takes

Implication

Neuroanatomical study uncovers synaptic targets and extrinsic catecholaminergic projections of tyrosine hydroxylase in rat parabrachial regions, suggesting intricate sensory processing networks.

Key Points

To determine the ultrastructural localization and extrinsic origins of the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH) within the lateral parabrachial region of adult male rats.
Examined acrolein-fixed coronal brain sections from adult male rats using electron microscopy and TH immunocytochemistry via the peroxidase-antiperoxidase method.
Combined TH immunolabeling with retrograde axonal tracing by unilaterally injecting wheat germ agglutinin-conjugated horseradish peroxidase (WGA-HRP) into the parabrachial region.
TH-immunoreactive perikarya and dendrites constituted only 17% of labeled profiles, mostly received noncatecholaminergic inputs, and localized primarily to the central lateral nucleus.
Labeled axons and axon terminals accounted for the remaining 83% of profiles, dividing into small Type I (0.3–0.6 µm) and large dense-core vesicle-containing Type II (0.8–1.4 µm) terminals morphologically resembling dopaminergic inputs.
Retrograde tracing confirmed that multiple extrinsic catecholaminergic cell groups project directly to the lateral parabrachial region, evidenced by dense WGA-HRP reaction product within TH-positive neurons.

Cite This Study

Milner et al. (1986) studied this question.

synapsesocial.com/papers/6a842716064c65267cc6bcbf https://doi.org/10.1523/jneurosci.06-09-02585.1986

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