Key result
Bovine arterial actomyosin possesses predominantly a myosin-associated calcium regulatory system, with calcium binding not fully accounted for by purified myosin alone.
This basic science study demonstrates that the calcium regulatory system in bovine arterial actomyosin is predominantly associated with the myosin filament.
Bovine arterial findings do not guide human vascular therapy; leaves open precise myosin-linked calcium regulation in mammalian smooth muscle.
A calcium-sensitive actomyosin was prepared from bovine aortic muscularis. The results of applying filament displacement tests indicate that bovine arterial actomyosin possesses predominantly a calcium regulatory system associated with the myosin filament, but also may possess a calcium regulatory system associated with the filament. Calcium binding to highly purified myosin preparations could not account for the calcium binding to calcium-sensitive actomyosin. A calcium-insensitive actomyosin preparation, prepared by omitting dithiothreitol from the isolation procedure, showed a definite loss of tropomyosin but no change in calcium binding relative to the calcium-sensitive actomyosin.
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Litten et al. (2008) studied this question. Filament displacement tests and calcium binding assays was evaluated on Calcium regulatory system characteristics. Bovine arterial actomyosin possesses predominantly a myosin-associated calcium regulatory system, with calcium binding not fully accounted for by purified myosin alone.
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