Key result
Five linked copies of the 9-nt Gtx IRES module enhanced translation efficiency in a monocistronic context by approximately 3-fold, with maximal enhancement observed with a 7-nt complementary match.
Effect estimate: ~3-fold
The Gtx 9-nt sequence enhances translation efficiency in eukaryotic cells via a mechanism involving base pairing to 18S rRNA.
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May aid preclinical gene expression tools in cardiovascular models; leaves open clinical translation.
Chappell et al. (2004) studied this question. 9-nt Gtx IRES module vs. Control constructs lacking the Gtx sequences was evaluated on Translation efficiency in a monocistronic context (~3-fold). Five linked copies of the 9-nt Gtx IRES module enhanced translation efficiency in a monocistronic context by approximately 3-fold, with maximal enhancement observed with a 7-nt complementary match.
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