Key result
Two nuclear localization signals (amino acids 4-7 and 90-96) mediate the nuclear import of Duck enteritis virus UL13 protein via a classical importin α/β-dependent process, but their deletion does not affect viral replication in vitro.
Why the study?
Although the UL13 multifunctional tegument protein of duck enteritis virus is predicted as a protein kinase, little was known concerning its subcellular localization signal.
DEV UL13 protein contains two functional nuclear localization signals that mediate its nuclear import via the classical importin α/β pathway, though this nuclear entry is not essential for viral replication in vitro.
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UL13 nuclear import is dispensable for DEV replication in vitro; leaves open its role in vivo or pathogenesis.
Background UL13 multifunctional tegument protein of duck enteritis virus (DEV) is predicted as protein kinase (CHPK); however, little is known concerning its subcellular localization signal. Results In this study, by transfection with two predicted nuclear signals of DEV UL13 fused to enhanced green fluorescent protein (EGFP), two bipartite nuclear localization signal (NLS) was identified. We identified the nuclear localization signals (NLSs) that control its nuclear importing using fluorescence assay and proved that nuclear localization of DEV UL13 is a classical importin α/β-dependent process. And we constructed the mutant DEV, with the NLSs of UL13 deleted, to explore whether it will affect the replication of virus particles. Conclusions DEV UL13 protein is directed by amino acids 4 to 7 and 90 to 96, and proved that this nuclear import occurs via the classical importin α/β-dependent process. And Entry nucleus of UL13 protein has no effect on DEV replication in cell culture. Our study enhances the understanding of DEV pUL13. Taken together, these results would provide significant information for the stud y of the biological function of UL13 during DEV infection.
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Yang et al. (2020) studied Duck enteritis virus (DEV) infection. Nuclear localization signal (NLS) deletion and ivermectin treatment vs. Wild-type DEV UL13 and untreated cells was evaluated on Intracellular localization of DEV UL13 protein and viral replication. Two nuclear localization signals (amino acids 4-7 and 90-96) mediate the nuclear import of Duck enteritis virus UL13 protein via a classical importin α/β-dependent process, but their deletion does not affect viral replication in vitro.
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