Key result
Yeast two-hybrid screening identified 57 host proteins that interact with both CSFV and BVDV E2 proteins, and two proteins that interact exclusively with CSFV E2.
The identification of 57 host proteins interacting with pestivirus E2 proteins provides a foundation for elucidating mechanisms of host protein-virus interactions during infection.
Hypothesis-generating for pestivirus E2-host interactions; functional validation in infection models required before mechanistic or therapeutic implications.
E2 is one of the envelope glycoproteins of pestiviruses, including classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV). E2 is involved in several critical functions, including virus entry into target cells, induction of a protective immune response and virulence in swine. However, there is no information regarding any host binding partners for the E2 proteins. Here, we utilized the yeast two-hybrid system and identified fifty-seven host proteins as positive binding partners which bound E2 from both CSFV and BVDV with the exception of two proteins that were found to be positive for binding only to CSFV E2. Alanine scanning of CSFV E2 demonstrated that the binding sites for these cellular proteins on E2 are likely non-linear binding sites. The possible roles of the identified host proteins are discussed as the results presented here will be important for future studies to elucidate mechanisms of host protein-virus interactions during pestivirus infection. However, due to the limitations of the yeast two hybrid system, the proteins identified is not exhaustive and each interaction identified needs to be confirmed by independent experimental approaches in the context of virus-infected cells before any definitive conclusion can be drawn on relevance for the virus life cycle.
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Gladue et al. (2014) studied Classical swine fever virus (CSFV) and bovine viral diarrhea virus (BVDV) infection. Yeast two-hybrid screening with CSFV and BVDV E2 proteins was evaluated on Identification of host proteins interacting with E2. Yeast two-hybrid screening identified 57 host proteins that interact with both CSFV and BVDV E2 proteins, and two proteins that interact exclusively with CSFV E2.
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