Rubella Complement Fixing Antigen Prepared by Alkaline Extraction Of Virus Grown in Suspension Culture of BHK-21 Cells

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SummaryAlkaline extracted rubella complement fixing antigen was prepared from infected BHK-21/13S cells grown in suspension culture. Although antigen potent enough to ensure optimal dilutions may not have been used, the complement fixing antibody titers of convalescent phase specimens were considerably higher than they were when commercial cell pack antigen was used. They were also higher than the titers reported earlier with the use of other types of rubella complement fixing antigens. The alkaline extracted antigens were stable at —70°C, —20°C, and +4°C.The excellent technical assistance of Miss Marianne Davis, Mrs. Carol Eason and Miss Ludie Holland is gratefully acknowledged.