Factors Affecting Recovery of Methyl Esters of Short-Chain Fatty Acids from Esterification Mixtures

volved hydrolysis to presumably the monoacid (since this was eliminated in the urine), but possibly the diacid metabolite. This conclusion was based on the fact that the Dacthal peak representing the entire amount of herbicide (50 ppm) resulted when each sample of liver extracted, with which Dacthal had been incubated, was evaporated and methylated and then chromatographed on the DC-200 column. Direct chromatography to identify the monoacid on Carbowax was impossible, because of a large interfering peak. Daethal incubated without liver did not decompose.