What question did this study set out to answer?

To determine the developmental role of the evolutionarily conserved T-box gene hrT in vertebrate cardiovascular morphogenesis.

November 1, 2002Development

hrTis required for cardiovascular development in zebrafish

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Authors

DSDaniel P. SzetoCalifornia Baptist University KGKevin GriffinCedars-Sinai Medical Center DKDavid KimelmanUniversity of Washington

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Implication

In vivo study reveals defective heart looping and impaired vasculogenesis in zebrafish lacking hrT, indicating its essential regulatory role in cardiovascular development.

Key Points

To determine the developmental role of the evolutionarily conserved T-box gene hrT in vertebrate cardiovascular morphogenesis.
Targeted gene knockdown of hrT in zebrafish embryos using morpholino antisense oligonucleotides.
Conducted hrT overexpression assays and evaluated heart looping, chamber morphology, circulation, and vascular gene expression patterns.
Zebrafish embryos lacking hrT displayed normal early heart assembly but subsequently failed heart looping, lacked blood circulation, and exhibited trunk vasculogenesis defects.
Loss of hrT function caused a dramatic upregulation of tbx5, whereas hrT overexpression significantly downregulated tbx5 expression.
HrT expression in vascular progenitors required midline mesoderm signaling, and hrT was required to inhibit expression of blood markers gata1 and gata2 in the posterior lateral plate mesoderm.

Cite This Study

Szeto et al. (2002) studied this question.

synapsesocial.com/papers/6a8536d3acfb9ccf0c9291af https://doi.org/10.1242/dev.129.21.5093

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Implication

In vivo study reveals defective heart looping and impaired vasculogenesis in zebrafish lacking hrT, indicating its essential regulatory role in cardiovascular development.

hrTis required for cardiovascular development in zebrafish

Discussion

Member takes

Implication

Key Points

Cite This Study

hrTis required for cardiovascular development in zebrafish

Discussion

Member takes

Implication

Key Points

Cite This Study

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