Why the study?
To review and update the occurrence of 2A/2A-like sequences across different groups of viruses.
The study expands the known distribution of 2A-like sequences across viral species, highlighting their evolutionary importance and potential biotechnological applications.
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Expands known 2A-like sequence distribution across RNA viruses; leaves open evolutionary and biotechnological roles.
2A is an oligopeptide sequence that mediates a ribosome "skipping" effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely distributed from insect to mammalian viruses and could act by accelerating adaptive capacity. These sequences have been used in many heterologous co-expression systems because they are versatile tools for cleaving proteins of biotechnological interest. In this work, we review and update the occurrence of 2A/2A-like sequences in different groups of viruses by screening the sequences available in the National Center for Biotechnology Information database. Interestingly, we reported the occurrence of 2A-like for the first time in 69 sequences. Among these, 62 corresponded to positive single-stranded RNA species, six to double stranded RNA viruses, and one to a negative-sense single-stranded RNA virus. The importance of these sequences for viral evolution and their potential in biotechnological applications are also discussed.
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Lima et al. (2021) studied this question.
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