The quantitative estimation of pyruvic and α-oxyglutaric acids by paper chromatography in blood, urine and cerebrospinal fluid

WORMALL I957sulphone and the amino groups of ac-amino acids proceeds exclusively via the f6rmation of two vinyl groups in the sulphone, followed by the addition of the amine to the ethylenic bonds.We are indebted to the British Empire Cancer Campaign for grants which have partly covered the cost of these investigations.We are also grateful to Dr T. E. Banks for advice and assistance inthe use and maintenance ofthe mass spectrometer and to Dr V. C. E. Burnop for help with one stage of the synthesis of deutero-mustard-gas sulphone and also for his assistance in some of the early investigations.The microanalyses were carried out by Miss J. Cuckney and the staff of the Microanalytical Laboratories of the Imperial College.