Proteomic profiling uncovers a three-protein biomarker panel in patient plasma, indicating strong diagnostic accuracy for abdominal aortic aneurysm detection.
Key Points
To identify and validate circulating plasma protein biomarkers for the noninvasive detection of abdominal aortic aneurysm.
Analyzed plasma from a discovery cohort of 22 patients with AAA and 22 healthy controls using data-independent acquisition mass spectrometry followed by bioinformatic enrichment analyses.
Validated candidate protein biomarkers via enzyme-linked immunosorbent assay in an independent cohort of 93 patients with AAA and 83 non-AAA controls.
Identified 111 differentially abundant proteins enriched in pathways for mitochondrial respiration, oxidative stress, inflammation, extracellular matrix remodeling, and proteostasis.
Confirmed significantly reduced plasma CHRDL1 and significantly elevated OGN and CCL18 levels in patients with AAA across both discovery and validation cohorts.
Demonstrated robust diagnostic capability using a combined three-protein panel (CHRDL1, OGN, CCL18), achieving an area under the receiver operating characteristic curve of 0.890.