Key result
Proteomics identifies 8 differentially regulated proteins in abdominal aortic aneurysm patients, including decreased bleomycin hydrolase.
Why the study?
Plasma biomarkers that identify abdominal aortic aneurysm rupture risk have not been successfully identified using previous methods.
Does tandem mass tag quantitative proteomics identify novel plasma biomarkers for abdominal aortic aneurysm compared to matched controls?
Case-Control (n=20)
Does tandem mass tag quantitative proteomics identify novel plasma biomarkers for abdominal aortic aneurysm compared to matched controls?
TMT-based proteomics identified novel potential plasma biomarkers, such as bleomycin hydrolase, for abdominal aortic aneurysm.
May support plasma proteomics for AAA biomarkers; hypothesis-generating and requires prospective validation.
Plasma biomarkers that identify abdominal aortic aneurysm (AAA) rupture risk would greatly assist in stratifying patients with small aneurysms. Identification of such biomarkers has hitherto been unsuccessful over a range of studies using different methods. The present study used an alternative proteomic approach to find new, potential plasma AAA biomarker candidates. Pre-fractionated plasma samples from twelve patients with AAA and eight matched controls without aneurysm were analyzed by mass spectrometry applying a tandem mass tag (TMT) technique. Eight proteins were differentially regulated in patients compared to controls, including decreased levels of the enzyme bleomycin hydrolase. The down-regulation of this enzyme was confirmed in an extended validation study using an enzyme-linked immunosorbent assay (ELISA). The TMT-based proteomic approach thus identified novel potential plasma biomarkers for AAA.
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Henriksson et al. (2018) conducted a case-control in Abdominal Aortic Aneurysm (n=20). Abdominal Aortic Aneurysm vs. Matched controls without aneurysm was evaluated on Differentially regulated plasma proteins. Tandem mass tag quantitative proteomics identified eight differentially regulated proteins in patients with abdominal aortic aneurysm compared to controls, including decreased bleomycin hydrolase.
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