Renal Vascular Response to Angiotensin II Administration in Two Kidneys-One Clip Hypertensive Rats Treated with High Dose of Estradiol: The Role of Mas Receptor

Backgrounds. High blood pressure is one of the most important causes of death around the world. The renin-angiotensin system (RAS) and estradiol are two important items that regulate arterial blood pressure in women. However, hypertension, RAS, and sex hormone estradiol may influence renal vascular responses. This study was designed to determine the role of Mas receptor (MasR) on renal vascular response to angiotensin II (Ang II) administration in two kidneys-one clip (2K1C) hypertensive rats treated with estradiol. Method. The ovariectomized rats were subjected to 2K1C or non-2K1C and simultaneously treated with estradiol (500 μg/kg/weekly) or placebo for a period of 4 weeks. Subsequently, under anesthesia, renal vascular responses to graded doses of Ang II administration with MasR blockade (A779) or its vehicle were determined. Results. A779 or its vehicle did not alter mean arterial pressure (MAP), renal perfusion pressure (RPP), and renal blood flow (RBF). However, in non-2K1C rats, Ang II infusion decreased RBF and increased renal vascular resistance (RVR) responses in a dose-related manner ( <a:math xmlns:a="http://www.w3.org/1998/Math/MathML" id="M1"> <a:mi>P</a:mi> <a:mtext>treat</a:mtext> <a:mo><</a:mo> <a:mn>0.0001</a:mn> </a:math> ). The greatest responses were found in ovariectomized estradiol-treated rats that received A779 ( <c:math xmlns:c="http://www.w3.org/1998/Math/MathML" id="M2"> <c:mi>P</c:mi> <c:mtext>group</c:mtext> <c:mo><</c:mo> <c:mn>0.05</c:mn> </c:math> ) in non-2K1C rats. Such findings were not detected in 2K1C hypertensive rats. For example, in estradiol-treated rats that received A779, at 1000 ng/kg/min of Ang II infusion, RBF reduced from <e:math xmlns:e="http://www.w3.org/1998/Math/MathML" id="M3"> <e:mn>1.6</e:mn> <e:mo>±</e:mo> <e:mn>0.2</e:mn> </e:math> to <g:math xmlns:g="http://www.w3.org/1998/Math/MathML" id="M4"> <g:mn>0.89</g:mn> <g:mo>±</g:mo> <g:mn>0.19</g:mn> </g:math> ml/min in non-2K1C rats, and it reduced from <i:math xmlns:i="http://www.w3.org/1998/Math/MathML" id="M5"> <i:mn>1.6</i:mn> <i:mo>±</i:mo> <i:mn>0.2</i:mn> </i:math> to <k:math xmlns:k="http://www.w3.org/1998/Math/MathML" id="M6"> <k:mn>1.2</k:mn> <k:mo>±</k:mo> <k:mn>0.2</k:mn> </k:math> ml/min in 2K1C rats. Conclusion. Hypertension induced by 2K1C may attenuate the role of A779 and estradiol in renal vascular responses to Ang II infusion. Perhaps, this response can be explained by the reduction of Ang II type 1 receptor (AT1R) expression in the 2K1C hypertensive rats.