Facet Dependence of Biosynthesis of Vivianite from Iron Oxides by Geobacter sulfurreducens

Vivianite plays an important role in alleviating the phosphorus crisis and phosphorus pollution. The dissimilatory iron reduction has been found to trigger the biosynthesis of vivianite in soil environments, but the mechanism behind this remains largely unexplored. Herein, by regulating the crystal surfaces of iron oxides, we explored the influence of different crystal surface structures on the synthesis of vivianite driven by microbial dissimilatory iron reduction. The results showed that different crystal faces significantly affect the reduction and dissolution of iron oxides by microorganisms and the subsequent formation of vivianite. In general, goethite is more easily reduced by Geobacter sulfurreducens than hematite. Compared with Hem₁₀₀ and Goe_L{110}, Hem₀₀₁ and Goe_H{110} have higher initial reduction rates (approximately 2.25 and 1.5 times, respectively) and final Fe(II) content (approximately 1.56 and 1.20 times, respectively). In addition, in the presence of sufficient PO43−, Fe(II) combined to produce phosphorus crystal products. The final phosphorus recoveries of Hem₀₀₁ and Goe_H{110} systems were about 5.2 and 13.6%, which were 1.3 and 1.6 times of those of Hem₁₀₀ and Goe_L{110}, respectively. Material characterization analyses indicated that these phosphorous crystal products are vivianite and that different iron oxide crystal surfaces significantly affected the size of the vivianite crystals. This study demonstrates that different crystal faces can affect the biological reduction dissolution of iron oxides and the secondary biological mineralization process driven by dissimilatory iron reduction.