July 1, 1985Proceedings of the National Academy of SciencesOpen Access

Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

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Population

HeLa cell nuclear extract

Design

Preclinical

Authors

HFHenry FurneauxAlbert Einstein College of Medicine KPK K PerkinsHoward Hughes Medical Institute GFG A FreyerColumbia University

Discussion

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Implication

Indicates RNA-dependent splicing as a therapeutic target; leaves open the component's identity and in vivo roles.

Structured PICO

Population

HeLa cell nuclear extract

Intervention

Separation by DEAE-cellulose chromatography into two fractions (Fractions I and II) and treatment with heat, N-ethylmaleimide, or micrococcal nuclease

Outcome

In vitro mRNA splicing activity of human beta-globin and adenovirus transcriptssurrogate

This basic science study identifies two distinct fractions from HeLa cells, one containing an essential nucleic acid component, that are required for in vitro mRNA splicing.

Cite This Study

Furneaux et al. (1985) studied this question.

synapsesocial.com/papers/6a8582498018c50ef23da701 https://doi.org/10.1073/pnas.82.13.4351

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Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider