What question did this study set out to answer?

To determine the cellular sources and mechanisms driving hydrogen peroxide-induced increases in intracellular calcium concentration within cardiomyocytes.

March 1, 1999Journal of Cardiovascular Pharmacology and Therapeutics

Mechanisms of Hydrogen Peroxide—Induced Increase in Intracellular Calcium in Cardiomyocytes

View Full Paper

Ask AI

Bookmark

Population

Cardiomyocytes isolated from adult male Sprague-Dawley rats and isolated perfused hearts

Comparison

Hydrogen peroxide (H2O2) 0.1-1 mmol/L vs Control conditions, or co-incubation with…

Design

Preclinical

Authors

XWXi WangNorth Sichuan Medical University STSatoshi TakedaJuntendo University Shizuoka Hospital SMSeibu MochizukiJikei University School of Medicine

Discussion

Member takes

Implication

H2O2-driven calcium overload may contribute to contractile dysfunction in cardiomyocytes; hypothesis-generating for PKC-targeted therapies, with no clinical implications yet.

Key Points

To determine the cellular sources and mechanisms driving hydrogen peroxide-induced increases in intracellular calcium concentration within cardiomyocytes.
Isolated cardiomyocytes and intact hearts from adult male Sprague-Dawley rats.
Measured intracellular calcium via Fura-2 fluorescence following exposure to 0.25 to 1.0 mmol/L hydrogen peroxide in the presence of various channel and enzyme inhibitors.
Hydrogen peroxide elevated intracellular calcium in a time- and concentration-dependent manner; this response was attenuated by catalase but unaffected by verapamil or extracellular calcium removal.
Inhibition of protein kinase C with staurosporine or sarcoplasmic reticulum calcium handling with thapsigargin and ryanodine markedly prevented the calcium increase without impacting cell viability.
Perfusion of isolated hearts with 0.1 to 0.5 mmol/L hydrogen peroxide impaired left ventricular developed pressure and contraction-relaxation rates while increasing end-diastolic pressure.

Structured PICO

Population

Cardiomyocytes isolated from adult male Sprague-Dawley rats and isolated perfused hearts

Intervention

Hydrogen peroxide (H2O2) 0.1-1 mmol/L

Comparator

Control conditions, or co-incubation with catalase, mannitol, verapamil, staurosporin, thapsigargin, or ryanodine

Outcome

Intracellular concentration of Ca2+ ([Ca2+]i) and left ventricular hemodynamics (developed pressure, rate of contraction, rate of relaxation, end-diastolic pressure)surrogate

High concentrations of hydrogen peroxide increase intracellular calcium in cardiomyocytes via a protein kinase C-mediated mechanism involving the sarcoplasmic reticulum, leading to contractile dysfunction.

Cite This Study

Wang et al. (1999) studied this question.

synapsesocial.com/papers/6a858fe7d1e5e0588dd4e534 https://doi.org/10.1177/107424849900400107

View Full Paper

Ask AI

Bookmark

Implication

H2O2-driven calcium overload may contribute to contractile dysfunction in cardiomyocytes; hypothesis-generating for PKC-targeted therapies, with no clinical implications yet.

Mechanisms of Hydrogen Peroxide—Induced Increase in Intracellular Calcium in Cardiomyocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Mechanisms of Hydrogen Peroxide—Induced Increase in Intracellular Calcium in Cardiomyocytes

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider