Repair of N-methyl-N'-nitro-N-nitrosoguanidine-induced DNA damage by ABC excinuclease
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Discussion
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Implication
Laboratory study demonstrates ABC excinuclease repairs nitrosoguanidine-induced DNA damage in Escherichia coli, indicating nucleotide excision repair targets minor helical lesions.
Key Points
- To determine whether Escherichia coli ABC excinuclease recognizes and repairs subtle DNA damage caused by the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine.
- Measured in vitro incising activity of reconstituted ABC excinuclease (encoded by uvrA, uvrB, and uvrC) on N-methyl-N'-nitro-N-nitrosoguanidine-treated DNA in the presence of ATP.
- Assessed in vivo DNA repair participation using various Escherichia coli alkylation repair-deficient mutant strains.
- Reconstituted ABC excinuclease incised methylated DNA in vitro in a dose-dependent, ATP-dependent manner.
- In vivo survival and repair patterns across alkylation repair-deficient mutants confirmed active excinuclease engagement in processing nitrosoguanidine-induced damage.
Cite This Study
Houten et al. (1987) studied this question.