Key result
A novel non-ribosomal random PCR and next-generation sequencing assay outperformed conventional random PCR, yielding higher percentages of viral reads (6% vs 0.9%) and correctly serotyping 100% of tested HFMD specimens.
Population
16 EV-A71/CV-A6/CV-A10/CV-A16 PCR positive rectal/throat swabs from hand, foot and mouth disease patients in…
Comparison
Non-ribosomal random PCR and next-generation… vs Conventional rPCR and direct sequencing
Design
Other
Authors
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May improve HFMD diagnostics from clinical samples; leaves open prospective validation before routine use.
Absolute Event Rate: 6% vs 0.9%
A novel non-ribosomal random PCR and next-generation sequencing assay successfully enables sensitive detection and direct whole-genome sequencing of HFMD pathogens from clinical samples.
Nguyen et al. (2016) studied Hand, foot and mouth disease (n=16). Non-ribosomal random PCR and next-generation sequencing assay vs. Conventional random PCR was evaluated on Percentage of EV-A71 reads (sample with Cp value 26). A novel non-ribosomal random PCR and next-generation sequencing assay outperformed conventional random PCR, yielding higher percentages of viral reads (6% vs 0.9%) and correctly serotyping 100% of tested HFMD specimens.
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