January 1, 2002Journal of Biological ChemistryOpen Access

Rho-dependent Agonist-induced Spatio-temporal Change in Myosin Phosphorylation in Smooth Muscle Cells

View Full Paper

Ask AI

Bookmark

Population

Differentiated tracheal smooth muscle cells

Comparison

Carbachol stimulation, with or without ML-9 or… vs Control cells

Design

Preclinical

Authors

KMKôji MiyazakiKyushu University TYTakeo YanoMie University DSDavid J. SchmidtUniversidade de Ribeirão Preto

Discussion

Member takes

Implication

Does not yet support clinical targeting; extends basic understanding of myosin regulation in smooth muscle.

Structured PICO

Population

Differentiated tracheal smooth muscle cells

Intervention

Carbachol stimulation, with or without ML-9 (myosin light chain kinase-specific inhibitor) or Y-27632 (Rho kinase-specific inhibitor)

Comparator

Control cells (for dominant negative RhoA experiments)

Outcome

Spatio-temporal change in myosin regulatory light chain (MLC20) phosphorylation and RhoA translocationsurrogate

This study demonstrates that the myosin light chain kinase and Rho pathways regulate myosin phosphorylation in smooth muscle cells through distinct spatio-temporal mechanisms.

Cite This Study

Miyazaki et al. (2002) studied this question.

synapsesocial.com/papers/6a85a99ab451cea71dae72e6 https://doi.org/10.1074/jbc.m108568200

View Full Paper

Ask AI

Bookmark

Rho-dependent Agonist-induced Spatio-temporal Change in Myosin Phosphorylation in Smooth Muscle Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Rho-dependent Agonist-induced Spatio-temporal Change in Myosin Phosphorylation in Smooth Muscle Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider