A standard test mixture for assessing enantioselectivity of chiral phase capillary GC columns – CHIRAL‐Test I for amide phases

A test mixture is proposed which allows assessment of the enantioselective separation potential of chiral amide phase capillary columns. This CHIRAL‐Test I includes a range of chromatographically problematical substrates, covering the temperature range from 100–200°C. One test component, the N ‐pivaloyl leucine methyl ester enatiomers, may be used exemplarily to calculate chiral resolution ( chirale Auflösung, cR ). Unlike separation factors α, cR values take account of peak shape as well as of relative retention time of both enantiomers, and thus are a measure of affective chromatographic separation. In combination with the Grob test, the CHIRAL‐Test may be used for characterizing the chromatographical properties of columns coated with chiral amide phases.