Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
December 1, 2001Journal of Biological ChemistryOpen Access

Critical Roles of a Cyclic AMP Responsive Element and an E-box in Regulation of Mouse Renin Gene Expression

View Full Paper
Ask AI
Bookmark
Share

Key result

A cyclic AMP responsive element (CRE) and an E-box are critical for synergistic activation of the mouse Ren-1(c) gene enhancer in As4.1 cells.

Population

Mouse As4.1 cells (renal renin expressing cells obtained after transgene-targeted oncogenesis)

Comparison

Transfection with renin promoter-luciferase… vs Wild-type constructs and untreated cells

Design

Preclinical

Authors

PLPan LiHandan CollegeQSQi ShiSouth China Normal UniversityCJCraig A. JonesIntegral Consulting (United States)

Discussion

Loading...

Member takes

Overview

Reveals key transcriptional regulators of mouse renin; leaves open applicability to human cardiovascular disease mechanisms or therapies.

Structured PICO

P
Population
Mouse As4.1 cells (renal renin expressing cells obtained after transgene-targeted oncogenesis)
I
Intervention
Transfection with renin promoter-luciferase reporter gene constructs (wild-type and mutated CRE/E-box) and treatment with protein kinase A inhibitor H-89
C
Comparator
Wild-type constructs and untreated cells
O
Outcome
Enhancer activity and Ren-1(c) gene expressionsurrogate

The CRE and E-box elements are critical for the synergistic activation and regulation of mouse Ren-1(c) gene expression.

Cite This Study

Li et al. (2001) studied this question. H-89 (protein kinase A inhibitor) and reporter gene constructs was evaluated on Enhancer activity and Ren-1(c) gene expression. A cyclic AMP responsive element (CRE) and an E-box are critical for synergistic activation of the mouse Ren-1(c) gene enhancer in As4.1 cells.

synapsesocial.com/papers/6a85b761f768ec88efd0d4bahttps://doi.org/10.1074/jbc.m103010200
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Differential activation of the SMαA promoter in smooth vs. skeletal muscle cells by bHLH factors1999 · 29 citations
  2. 2Induction of proto-oncogene fos transcription through the adenylate cyclase pathway: characterization of a cAMP-responsive element.1988 · 386 citations
  3. 3Conserved Enhancer Elements in Human and Mouse Renin Genes Have Different Transcriptional Effects in As4.1 Cells1997 · 59 citations
  4. 4Expression of a renin/GFP transgene in mouse embryonic, extra-embryonic, and adult tissues2000 · 63 citations
  5. 5Expression of Murine Renin Genes during Fetal Development1990 · 118 citations