A system for rapid hemoglobin electrophoresis on both cellulose acetate and citrate-agar combined with a simple solubility test for sickling variants is described. By use of a saponin hemolysate of once-washed erythrocytes, all three tests can be performed at one time so that an accurate result of the hemoglobin phenotype can be provided expeditiously. A technic for rapid hemoglobin electrophoresis on citrate-agar, using standard equipment is described in detail.
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Milner et al. (1975) studied this question.