What question did this study set out to answer?

To assess the specificity and reliability of various cellular, metabolic, and morphologic changes as indicators of in vitro neoplastic conversion induced by polyoma virus.

October 1, 1967JNCI Journal of the National Cancer Institute

Search for “Indicators” of Neoplastic Conversion In Vitro

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Authors

KSKatherine K. SanfordNational Cancer Institute BBBarbara E. BarkerProvidence College MWMark WoodsDefense Logistics Agency

Discussion

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Overview

In vitro study reveals cytologic features serve as more specific indicators of neoplastic conversion than morphologic patterns in rodent cells, suggesting revised criteria for transformation.

Key Points

To assess the specificity and reliability of various cellular, metabolic, and morphologic changes as indicators of in vitro neoplastic conversion induced by polyoma virus.
Examined cultured mouse and hamster cells infected with polyoma virus across short-term, slow-growing, rapidly growing, and prolonged culture conditions.
Evaluated candidate conversion markers, including transplantation antigens, cell morphology, cytology, karyology, proliferation rates, and glycolytic activities alongside uninfected controls.
Morphologic growth patterns, elevated proliferation rates, and increased glycolytic activity correlated with neoplastic conversion only in short-term, slow-growing cultures, but also appeared in non-neoplastic long-term cultures.
Cytologic alterations in virus-treated cells closely resembled features of in vivo neoplasms, indicating potential utility as sensitive markers of neoplastic transformation.
Altered morphologic growth patterns reflected reduced substrate adhesion and accelerated proliferation rather than a genuine loss of contact inhibition.

Cite This Study

Sanford et al. (1967) studied this question.

synapsesocial.com/papers/6a86683ef5121d64a72ed726 https://doi.org/10.1093/jnci/39.4.705

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October 1, 1967JNCI Journal of the National Cancer Institute

Search for “Indicators” of Neoplastic Conversion In Vitro

View Full Paper

Ask AI

Bookmark

Authors

KSKatherine K. SanfordNational Cancer Institute BBBarbara E. BarkerProvidence College MWMark WoodsDefense Logistics Agency

Discussion

Member takes

Overview

In vitro study reveals cytologic features serve as more specific indicators of neoplastic conversion than morphologic patterns in rodent cells, suggesting revised criteria for transformation.

Key Points

To assess the specificity and reliability of various cellular, metabolic, and morphologic changes as indicators of in vitro neoplastic conversion induced by polyoma virus.
Examined cultured mouse and hamster cells infected with polyoma virus across short-term, slow-growing, rapidly growing, and prolonged culture conditions.
Evaluated candidate conversion markers, including transplantation antigens, cell morphology, cytology, karyology, proliferation rates, and glycolytic activities alongside uninfected controls.
Morphologic growth patterns, elevated proliferation rates, and increased glycolytic activity correlated with neoplastic conversion only in short-term, slow-growing cultures, but also appeared in non-neoplastic long-term cultures.
Cytologic alterations in virus-treated cells closely resembled features of in vivo neoplasms, indicating potential utility as sensitive markers of neoplastic transformation.
Altered morphologic growth patterns reflected reduced substrate adhesion and accelerated proliferation rather than a genuine loss of contact inhibition.

Cite This Study

Sanford et al. (1967) studied this question.

synapsesocial.com/papers/6a86683ef5121d64a72ed726 https://doi.org/10.1093/jnci/39.4.705

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