Why the study?
Does Calcium ionophore A23187 evoke and potentiate the antiviral activity of interferon in virus-infected cell lines?
Does Calcium ionophore A23187 evoke and potentiate the antiviral activity of interferon in virus-infected cell lines?
Calcium ionophore A23187 evokes antiviral activity and potentiates interferon against SBV and VSV, suggesting intracellular calcium changes play a role in interferon's antiviral mechanism.
Implicates calcium in interferon antiviral mechanisms; hypothesis-generating for adjunctive therapies and needs in vivo validation.
Calcium ionophore A23187, which causes a rapid efflux of Ca2+ from cells, evokes an antiviral response in mouse LB, simian COS-1, Hela, human amniotic (U), baby hamster kidney (BHK), and VERO cells against Sindbis (SBV) and vesicular stomatitis (VSV) viruses. The degree of antiviral activity depends on the type of cell, virus, and the dose of A23187. A23187 inhibits the production of infectious VSV; however, VSV particle production was not significantly inhibited as measured by viral RNA and viral proteins. The VSV released from the A23187-treated cells is deficient in VSV glycoprotein (G) and membrane (M) protein. A23187 potentiates the antiviral activity of interferon (IFN) against SBV and VSV in mouse LB and human U cells. It is possible to postulate that a change in intracellular Ca2+ may play an important role in the antiviral activity of IFN.
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Maheshwari et al. (1985) studied this question.
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