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February 1, 1993Journal of Biological ChemistryOpen Access

Molecular characterization and regulation of the human endothelin receptors.

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Population

Human cDNAs expressed in COS cells, Chinese hamster ovary cells, and Xenopus oocytes; marmosets maintained…

Comparison

Cloning and expression of human ETAR and ETBR… vs Rat ETAR and ETBR

Design

Preclinical

Authors

NENabil A. ElshourbagyNorthwestern UniversityDKDavid R. KormanNew FrontierHWH.L. WuStanford University

Discussion

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Implication

Human-rat ETAR pharmacological differences warrant species-specific validation; leaves open diet effects on primate ETBR for clinical translation.

Structured PICO

P
Population
Human cDNAs expressed in COS cells, Chinese hamster ovary cells, and Xenopus oocytes; marmosets maintained on a high fat/high cholesterol diet
I
Intervention
Cloning and expression of human ETAR and ETBR; high fat/high cholesterol diet in marmosets
C
Comparator
Rat ETAR and ETBR (for sequence and pharmacological comparison)
O
Outcome
Receptor sequence identity, pharmacological binding properties, electrophysiological function, and mRNA expression levelssurrogate

The cloning and characterization of human endothelin receptors reveal pharmacological differences from rat receptors and demonstrate diet-induced regulation of ETBR mRNA in a primate model.

Cite This Study

Elshourbagy et al. (1993) studied this question.

synapsesocial.com/papers/6a86b0023f44447e627cabe7https://doi.org/10.1016/s0021-9258(18)53554-7
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Also Consider

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  1. 1Autoradiographical Localization of Binding Sites for Porcine [125I]Endothelin-1 in Humans, Pigs, and Rats1989 · 182 citations
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  3. 3Endothelin stimulates c‐fos and c‐myc expression and proliferation of vascular smooth muscle cells1988 · 733 citations
  4. 4Dexamethasone down-regulates the expression of endothelin receptors in vascular smooth muscle cells.1992 · 45 citations
  5. 5RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination1977 · 3,485 citations