What question did this study set out to answer?

Address technical constraints and common failure modes in surface plasmon resonance imaging to reliably measure complex multiprotein assembly kinetics.

August 20, 2026Analytical ChemistryOpen Access

Challenges and Solutions in Developing Label-Free Optical Chip Based on Surface Plasmon Resonance Imaging (SPRi) for Multiprotein Assembly Sensing

View Full Paper

Ask AI

Bookmark

Authors

EVEdita VoitechovičCenter for Physical Sciences and Technology TRTomas RakickasCenter for Physical Sciences and Technology LSLiudmyla ShmelevaTaras Shevchenko National University of Kyiv

Discussion

Member takes

Implication

In vitro study demonstrates an optimized phase-sensitive surface plasmon resonance imaging platform, indicating improved kinetic accuracy and range for detecting multiprotein assemblies.

Key Points

Address technical constraints and common failure modes in surface plasmon resonance imaging to reliably measure complex multiprotein assembly kinetics.
Implemented a null-ellipsometry phase-interrogation surface plasmon resonance imaging (SPRi) system utilizing 658 nm illumination at an incidence angle near 60°.
Patterned protein-repellent and functionalized capture regions on sensor chips using microcontact printing.
Fabricated a custom microfluidic flow cell and a multilayer dielectric substrate to manage mass-transport limitations and expand dynamic measurement range.
Microcontact-printed surface functionalization effectively suppressed nonspecific binding across immunoassay domains.
Custom microfluidic cell accelerated fluid exchange, eliminating mass-transport artifacts that typically distort kinetic rate constants.
Multilayer dielectric chip configuration successfully extended the detectable thickness limit for large multiprotein assemblies at a fixed angle of incidence.

Cite This Study

Voitechovič et al. (2026) studied this question.

synapsesocial.com/papers/6a86b5978a91293e6a1ccff7 https://doi.org/10.1021/acs.analchem.6c00020

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates an optimized phase-sensitive surface plasmon resonance imaging platform, indicating improved kinetic accuracy and range for detecting multiprotein assemblies.

Challenges and Solutions in Developing Label-Free Optical Chip Based on Surface Plasmon Resonance Imaging (SPRi) for Multiprotein Assembly Sensing

Discussion

Member takes

Implication

Key Points

Cite This Study

Challenges and Solutions in Developing Label-Free Optical Chip Based on Surface Plasmon Resonance Imaging (SPRi) for Multiprotein Assembly Sensing

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider