What question did this study set out to answer?

To evaluate the effects of rosiglitazone on insulin-stimulated PKC-zeta/lambda activation and glucose transport in adipocytes from diabetic and nondiabetic rats.

June 1, 2000Journal of Biological ChemistryOpen Access

Thiazolidinedione Treatment Enhances Insulin Effects on Protein Kinase C-ζ/λ Activation and Glucose Transport in Adipocytes of Nondiabetic and Goto-Kakizaki Type II Diabetic Rats

View Full Paper

Ask AI

Bookmark

Authors

YKYoshinori KanohChildren's Medical Center GBGautam BandyopadhyayNational Institute of Technology Durgapur MSMini P. SajanUnited States Department of Veterans Affairs

Discussion

Member takes

Implication

Preclinical study demonstrates rosiglitazone enhances insulin-induced glucose uptake via PKC-zeta/lambda activation in rat adipocytes, highlighting a mechanism for improving insulin action.

Key Points

To evaluate the effects of rosiglitazone on insulin-stimulated PKC-zeta/lambda activation and glucose transport in adipocytes from diabetic and nondiabetic rats.
Isolated adipocytes from Goto-Kakizaki (GK) type II diabetic rats and nondiabetic control rats.
Treated adipocyte preparations with rosiglitazone and measured insulin-stimulated 2-deoxyglucose uptake.
Assessed enzyme activity and phosphorylation sites of PKC-zeta/lambda, IRS-1-dependent PI 3-kinase, and protein kinase B (PKB).
Insulin-stimulated 2-deoxyglucose uptake and PKC-zeta/lambda activity were diminished by approximately 50% in GK adipocytes compared to control adipocytes.
Rosiglitazone completely reversed defects in 2-deoxyglucose uptake, PKC-zeta/lambda activity, and PKC-zeta Thr-410 phosphorylation in GK adipocytes.
Rosiglitazone enhanced glucose uptake and PKC-zeta/lambda activation in nondiabetic adipocytes without altering PI 3-kinase or PKB activation in either group.

Cite This Study

Kanoh et al. (2000) studied this question.

synapsesocial.com/papers/6a86d565972878eab4beec8b https://doi.org/10.1074/jbc.m000287200

View Full Paper

Ask AI

Bookmark

Implication

Preclinical study demonstrates rosiglitazone enhances insulin-induced glucose uptake via PKC-zeta/lambda activation in rat adipocytes, highlighting a mechanism for improving insulin action.

Thiazolidinedione Treatment Enhances Insulin Effects on Protein Kinase C-ζ/λ Activation and Glucose Transport in Adipocytes of Nondiabetic and Goto-Kakizaki Type II Diabetic Rats

Discussion

Member takes

Implication

Key Points

Cite This Study

Thiazolidinedione Treatment Enhances Insulin Effects on Protein Kinase C-ζ/λ Activation and Glucose Transport in Adipocytes of Nondiabetic and Goto-Kakizaki Type II Diabetic Rats

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider