Key result
A single intraperitoneal injection of monocrotaline in young Tibet minipigs increased mean pulmonary arterial pressure compared to placebo at 6 weeks.
Why the study?
Does a single intraperitoneal injection of monocrotaline establish a chronic pulmonary hypertension model in young Tibet minipigs?
Does a single intraperitoneal injection of monocrotaline establish a chronic pulmonary hypertension model in young Tibet minipigs?
A single intraperitoneal injection of monocrotaline successfully establishes a stable, large animal model of chronic pulmonary hypertension in young Tibet minipigs, verifiable by both invasive and noninvasive methods.
Answers are grounded in this paper and cite their sources.
No immediate clinical impact; extends large-animal options for pulmonary hypertension research but leaves translation open.
OBJECTIVE: The purpose of this study was to establish an animal model of chronic pulmonary hypertension with a single-dose intraperitoneal injection of monocrotaline (MCT) in young Tibet minipigs, so as to enable both invasive and noninvasive measurements and hence facilitate future studies. METHODS: Twenty-four minipigs (8-week-old) were randomized to receive single-dose injection of 12.0 mg/kg MCT (MCT group, n = 12) or placebo (control group, n = 12 each). On day 42, all animals were evaluated for pulmonary hypertension with conventional transthoracic echocardiography, right heart catheterization (RHC), and pathological changes. Findings of these studies were compared between the two groups. RESULTS: At echocardiography, the MCT group showed significantly higher pulmonary arterial mean pressure (PAMP) compared with the controls (P<0.001). The pulmonary valve curve showed v-shaped signals with reduction of a-waves in minipigs treated with MCT. In addition, the MCT group had longer pulmonary artery pre-ejection phases, and shorter acceleration time and ejection time. RHC revealed higher mean pulmonary arterial pressure (mPAP) in the MCT group than in the control group (P<0.01). A significant and positive correlation between the mPAP values and the PAMP values (R = 0.974, P<0.0001), and a negative correlation between the mPAP and ejection time (R = 0.680, P<0.0001) was noted. Pathology demonstrated evidence of pulmonary vascular remodeling and higer index of right ventricular hypertrophy in MCT-treated minipigs. CONCLUSION: A chronic pulmonary hypertension model can be successfully established in young minipigs at six weeks after MCT injection. These minipig models exhibited features of pulmonary arterial hypertension that can be evaluated by both invasive (RHC) and noninvasive (echocardiography) measurements, and may be used as an easy and stable tool for future studies on pulmonary hypertension.
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Zeng et al. (2013) studied Chronic pulmonary hypertension (n=24). Monocrotaline vs. Placebo (75% alcohol) was evaluated on Mean pulmonary arterial pressure (mPAP) measured by right heart catheterization at 42 days (p=<0.01). A single intraperitoneal injection of monocrotaline in young Tibet minipigs increased mean pulmonary arterial pressure compared to placebo at 6 weeks.
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