Key result
In vivo gene transfer of a short hairpin RNA targeting the L-type calcium channel accessory beta-subunit attenuated the hypertrophic response in a rat model without compromising systolic performance.
Why the study?
Does genetic suppression of the L-type calcium channel accessory beta-subunit reduce cardiac hypertrophy in rat models?
Population
Neonatal rat cardiomyocytes and an aortic-banded rat model of left ventricular hypertrophy
Comparison
Lentiviral vector (PPT.CG.H1.beta) delivering… vs Nonsilencing controls
Design
Preclinical
Follow-up
4 weeks post transduction (for in vivo model)
Authors
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Hypothesis-generating in rat hypertrophy models; should not change practice pending human translation.
Does genetic suppression of the L-type calcium channel accessory beta-subunit reduce cardiac hypertrophy in rat models?
Genetic suppression of the L-type calcium channel beta subunit attenuates cardiac hypertrophy in rat models without compromising systolic function, suggesting a potential novel therapeutic strategy.
Cingolani et al. (2007) studied Left ventricular hypertrophy. Lentiviral vector expressing short hairpin RNA against L-type calcium channel accessory beta-subunit (PPT.CG.H1.beta(2)) vs. Nonsilencing controls was evaluated on Hypertrophic response (left ventricular wall thickness and heart weight/body weight ratios). In vivo gene transfer of a short hairpin RNA targeting the L-type calcium channel accessory beta-subunit attenuated the hypertrophic response in a rat model without compromising systolic performance.
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