November 15, 2016PLoS ONEOpen Access

Interaction between the Stress Phase Angle (SPA) and the Oscillatory Shear Index (OSI) Affects Endothelial Cell Gene Expression

Q: What is the clinical evidence from this study?

Study design: Other. Population: Atherosclerosis (in vitro model). Intervention: Asynchronous hemodynamics (SPA = -180°) with reversing flow (OSI = 0.15) vs. Synchronous hemodynamics (SPA = 0°) with reversing flow (OSI = 0.15). Primary outcome: Endothelial cell gene expression (42 atherosclerosis-related genes).

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Key result

Asynchronous hemodynamics combined with reversing flow upregulated 8 genes, mostly proatherogenic, compared to synchronous hemodynamics with reversing flow in endothelial cells.

Population

Bovine aortic endothelial cells (BAECs) between passages 3 and 7 seeded onto silicone membranes

Comparison

Asynchronous hemodynamics with reversing flow… vs Synchronous hemodynamics with and without…

Design

Preclinical

Follow-up

7 hours

Authors

RARonny AmayaCity College of New York LCLimary M. CancelCity College of New York JTJohn M. TarbellCross-Cutting Cardiology

Discussion

Member takes

Implication

Hypothesis-generating in endothelial models; leaves open clinical relevance of asynchronous flow to human atherosclerosis and needs human validation.

Structured PICO

Population

In vitro study using Bovine Aortic Endothelial Cells to evaluate the effects of hemodynamic forces on atherosclerosis-related gene expression.

Intervention

Asynchronous hemodynamics (stress phase angle [SPA] = -180°) with reversing flow (oscillatory shear index [OSI] = 0.15) applied for 7 hours

Comparator

Synchronous hemodynamics (SPA = 0°) with and without reversing flow, asynchronous hemodynamics without reversing flow, and static control

Outcome

Expression of 37-42 atherosclerosis-related genes and protein expression/nuclear translocation of NFκB p65surrogate

Asynchronous hemodynamics and reversing flow synergistically elicit proatherogenic gene expression and NFκB activation in endothelial cells, highlighting their role in atherosclerosis localization.

Limitations

In vitro study design
Future experiments are needed to examine the in vivo roles of the SPA in vascular biological responses

Cite This Study

Amaya et al. (2016) studied Atherosclerosis (in vitro model). Asynchronous hemodynamics (SPA = -180°) with reversing flow (OSI = 0.15) vs. Synchronous hemodynamics (SPA = 0°) with reversing flow (OSI = 0.15) was evaluated on Endothelial cell gene expression (42 atherosclerosis-related genes). Asynchronous hemodynamics combined with reversing flow upregulated 8 genes, mostly proatherogenic, compared to synchronous hemodynamics with reversing flow in endothelial cells.

synapsesocial.com/papers/6a86f23652b690c5299eb22a https://doi.org/10.1371/journal.pone.0166569

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Implication

Hypothesis-generating in endothelial models; leaves open clinical relevance of asynchronous flow to human atherosclerosis and needs human validation.

Interaction between the Stress Phase Angle (SPA) and the Oscillatory Shear Index (OSI) Affects Endothelial Cell Gene Expression

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Interaction between the Stress Phase Angle (SPA) and the Oscillatory Shear Index (OSI) Affects Endothelial Cell Gene Expression

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider