Key result
Mutation of the GYG signature sequence in murine Kir2.1 channels did not alter ionic selectivity but significantly reduced channel open times, indicating the H5 region helps determine open state dwell time.
Population
Murine Kir2.1 channels
Comparison
Mutations replacing Tyr in the GYG signature… vs Wild-type murine Kir2.1 channels
Design
Preclinical
Authors
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Hypothesis-generating for Kir channel gating; does not support changes in clinical arrhythmia management.
In murine Kir2.1 channels, the GYG signature sequence can tolerate mutations for K+ selectivity, but the specific residue at position 145 is critical for determining channel open time duration.
So et al. (2001) studied this question. Mutation of Tyr (Y145) in the GYG signature sequence vs. Wild-type Kir2.1 was evaluated on Ionic selectivity and gating (channel open times, unitary conductance). Mutation of the GYG signature sequence in murine Kir2.1 channels did not alter ionic selectivity but significantly reduced channel open times, indicating the H5 region helps determine open state dwell time.
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