Key result
Expression of dominant-negative KCNQ3 reduced M-current density in rat sympathetic neurons by 47%, whereas dominant-negative Merg1a had no effect, delineating their specific channel contributions.
Pore-defective mutants of Merg1a and KCNQ3 effectively act as dominant-negative knock-downs to delineate the specific contributions of erg and KCNQ channel families to M-like potassium currents.
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Dominant-negative mutants distinguish KCNQ from erg contributions to neuronal M-currents; leaves open translation to intact ganglia or human physiology.
Selyanko et al. (2002) studied this question. Dominant-negative mutants of KCNQ3 (DN-KCNQ3) and Merg1a (DN-Merg1a) vs. CD8 alone or uninjected cells was evaluated on M-like potassium current amplitude and deactivation components. Expression of dominant-negative KCNQ3 reduced M-current density in rat sympathetic neurons by 47%, whereas dominant-negative Merg1a had no effect, delineating their specific channel contributions.
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