A Role for Periostin Pathological Variants and Their Interaction with HSP70-1a in Promoting Pancreatic Cancer Progression and Chemoresistance

Figures

• 

  Figure 1. The expression of total Pn is elevated in PDAC. (A) Representative Pn immunohistochemical staining of normal pancreas and PDAC. S: stroma, C: cancer cells. (B) % of Pn-positive area in PDAC (n = 20) and normal pancreas (n = 4). (C) Average % of Pn-positive area in PDAC and normal pancreas. * p < 0.05 vs. normal pancreas.

• 

  Figure 2. Pn-ASVs expression in PDAC and CAFs. (A) Human Pn-ASV structures. In addition to four major variants, Pn 1, Pn 2-1, Pn 3 and Pn 4-1, four other isoforms have been reported. Red arrows indicate the position of primers for Pn-ASV detection. The EMI domain, the four FAS-1 domains and the N- and C-terminal end of the carboxyl-terminal domain are depicted. (B) Pn-ASVs mRNA expression in PDAC cell lines (Panc 1, AsPC 1 and BxPC 3) and CAFs (hPSC5 and hPSC14). N = 4, * p < 0.05 vs. Panc 1, AsPC 1 and BxPC 3, ** p < 0.05 vs. Panc 1, AsPC 1, BxPC 3 and hPSC5. † p < 0.05 vs. Pn 1, Pn 3 and Pn 4, ‡ p < 0.05 vs. Pn 1 and Pn 2-1 and Pn 3. (C) Total Pn protein secreted from PDAC cell lines (Panc 1, AsPC 1 and BxPC 3) and CAFs (hPSC5 and hPSC14). (D) Pn-ASVs protein secreted from CAF. Pn-ASV proteins immunoprecipitated from a culture supernatant of CAFs using the specific antibodies against Pn exon 1, 17 and 21 were analyzed by Western blotting with Pn antibody for exon 12.

• 

  Figure 3. Restricted distribution of Pn-ASVs with exon 21 and sequencing analysis of Pn-positive cells. (A) A representative in situ hybridization image of total Pn (RNAscope) and Pn-ASVs with exon 21 (Basescope) in PDAC specimen. The red color indicates a positive signal for Pn mRNA. Both total Pn and Pn-ASVs with exon 21 mRNA was expressed in fibroblasts surrounding cancer. S: stroma, C: cancer cells. Bar indicates 100 μm. (B) Pn expression and prognosis in patients with PDAC. Pn expression and PDAC prognostic analysis was examined using the Kaplan–Meier plotter. It was found that the prognosis was worse in the group with high Pn expression. (C) Single-cell RNA-sequence analysis in different cell types show that the fibroblast and smooth muscle cell clusters are the ones that show high Pn expression. (D) Violin plot for Pn expression in different cell types in PDAC on the data from the Single Cell Portal.

• 

  Figure 4. PDAC tumors activate Pn expression in CAFs. (A) KPC mice-derived YFP+ PDAC cells were subcutaneously implanted in the back of 8-week-old male C57BL6 mice and sacrificed on day 35. (B) YFP-positive and negative cells isolation by fluorescence-activated cell sorting (FACS). (C) Total Pn (amplicon: exon 9–10) and Pn-ASV with exon 21 (amplicon: exon 21–22) expression was higher in YFP-negative cells containing CAFs. N = 4, * p < 0.05 vs. pre-transplantation cancer cell and YFP+ cell. (D) YFP+ PDAC cells were subcutaneously implanted into the back of 8-week-old male Postn-tdTomato mice and sacrificed on day 35. Tamoxifen was administered intraperitoneally 5 days prior to sacrifice. (E) Tdtomato, a Pn-positive signal, was identified in the stroma of the excised tumor. TFP-positive ODAC cells were shown in green. White bar indicates 100 μm. (F) CAFs were isolated from excised tumors by FACS using GFP-negative and CD90-positive sorting. Tdtomato was further used to divide the CAFs to Pn-positive and negative groups. (G) Tdtomato-positive CAFs had higher expression of total Pn (exon 9–10 amplicon) and exon 21-containing ASVs (exon 21–22 amplicon). N = 3, * p < 0.05 vs. CD90+ Tdtomato-CAFs.

• 

  Figure 5. The transcriptome of Pn-positive (CD90+ tdTomato+) and negative (CD90+ tdTomato-) CAFs. RNA sequence analysis was performed using Pn-positive and Pn-negative CAFs isolated from a mouse PDAC syngeneic model. RNA-seq analysis comparing Pn-positive and Pn-negative CAFs. (A) RNA-seq analysis showing a volcano plot and heat map of differentially expressed genes (DEGs) in Pn-positive and Pn-negative CAFs. The data represent three biological replicates. It revealed 7624 differentially expressed genes (DEGs, red dots in MA plot) when comparing Pn-positive CAFs from Pn-negative CAFs (FDR < 0.05). Among 7624 DEGs, 4418 were up-regulated, while 3206 were up-regulated in Pn-positive CAFs. (B) Gene ontology enrichment and KEGG analysis of up-regulated genes in the Pn-positive CAFs from the RNA-seq data. (C) Representative genes whose expression was increased in the Pn-positive CAFs group. Up-regulated genes in Pn-positive CAFs include several genes related to cancer progression and chemoresistance. Data are the log2 of fold change (LogFC). Relative expression pattern analysis of up-regulated genes in Pn-positive CAFs by qRT-PCR analysis to validate the RNA-seq data is shown in Supplementary…

• 

  Figure 6. Pn-ASVs with exon 21 interacts with HSP70 and promotes gemcitabine resistance in pancreatic cancer. (A) Pull-down solutions were electrophoresed on gels and silver stained. Protein analysis of bands that appeared specifically in the pull-down solution of Pn2-1 was performed (red arrows). (B) Amino acid sequence of HSP70 was detected by LC-MS/MS analysis of gel bands analysis. Western blotting with primary antibody for HSP70 in post-pull-down solution detected a strong band in lane Pn2-1 but not Pn4-1. (C) HSPA1A and HSPA8 expression in PDAC cells and CAFs. HSP70 was higher in PDAC cell lines as compared to CAFs. N = 4, * p < 0.05 vs. hPSC 5, ** p < 0.05 vs. BxPC 3. (D) The 3D predicted steric structures of Pn2-1 and Pn4-1, and predicted binding sites with HSPA1A are shown. Colored blue to red from n-terminus to c-terminus. (E) GEM significantly reducing proliferation of PDAC and CAFs cell lines. N = 6, * p < 0.05 vs. 0 μM. (F) Pn-ASV with exon 21 suppresses GEM-induced PDAC cell line cell death. However, knockdown of HSPA1A prevents the rescue benefit. N = 12, * p < 0.05 vs. CTRL siRNA.