Key result
Millimolar Mg2+ and Ca2+ concentrations interact with low affinity, nonselective divalent cation binding sites to shift BK channel activation primarily by slowing deactivation.
Population
Cloned mouse Slo1 alpha subunits (BK channels)
Comparison
Exposure to cytosolic calcium and magnesium at… vs Different concentrations of Ca2+ and Mg2+…
Design
Preclinical
Authors
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Extends models of BK channel gating by low-affinity sites; leaves open physiological or therapeutic relevance in humans.
BK channel activation across a broad range of calcium concentrations is mediated by multiple types of divalent cation binding sites, including a low-affinity nonselective site.
Zhang et al. (2001) studied this question. Ca2+ and Mg2+ vs. Lower concentrations or 0 Ca2+ was evaluated on Half-activation voltage (V0.5) and activation/deactivation rates. Millimolar Mg2+ and Ca2+ concentrations interact with low affinity, nonselective divalent cation binding sites to shift BK channel activation primarily by slowing deactivation.
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