What question did this study set out to answer?

To determine whether transforming growth factor-beta 1 modulates epidermal growth factor-stimulated phosphatidylinositol 3-kinase activation in human airway smooth muscle cells.

December 1, 1997AJP Lung Cellular and Molecular Physiology

TGF-β1 modulates EGF-stimulated phosphatidylinositol 3-kinase activity in human airway smooth muscle cells

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Authors

VKVera P. KrymskayaUniversity of Pennsylvania RHRebecca HoffmanGeisinger Health System AEAndrew EszterhasUniversity of Kentucky

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Implication

In vitro study demonstrates that TGF-beta 1 enhances EGF-induced PI 3-kinase activation in human airway smooth muscle cells, indicating novel crosstalk between growth factor receptor pathways.

Key Points

To determine whether transforming growth factor-beta 1 modulates epidermal growth factor-stimulated phosphatidylinositol 3-kinase activation in human airway smooth muscle cells.
Treated cultured human airway smooth muscle (hASM) cells with epidermal growth factor (EGF), transforming growth factor-beta 1 (TGF-beta 1), or a combination of both.
Assayed PI 3-kinase activation, association with phosphotyrosine proteins, p70 S6 kinase activity, and TGF-beta type I and II receptor co-immunoprecipitation.
Assessed cell cycle progression and myocyte apoptosis using cell cycle analysis and DNA ladder assays.
EGF alone induced a 5.7 ± 1.2-fold activation of PI 3-kinase compared with diluent-treated controls, whereas TGF-beta 1 pretreatment combined with EGF markedly boosted PI 3-kinase activation to a 16.6 ± 1.9-fold increase.
PI 3-kinase associated with both type I and type II TGF-beta receptors, and TGF-beta 1 selectively enhanced PI 3-kinase activity associated with the type I receptor.
TGF-beta 1 did not alter EGF-induced p70 S6 kinase activity or PI 3-kinase binding to tyrosine-phosphorylated proteins, and neither TGF-beta 1 exposure nor PI 3-kinase inhibition triggered myocyte apoptosis.

Cite This Study

Krymskaya et al. (1997) studied this question.

synapsesocial.com/papers/6a871866a6ddb97d43d3d007 https://doi.org/10.1152/ajplung.1997.273.6.l1220

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Implication

In vitro study demonstrates that TGF-beta 1 enhances EGF-induced PI 3-kinase activation in human airway smooth muscle cells, indicating novel crosstalk between growth factor receptor pathways.

TGF-β1 modulates EGF-stimulated phosphatidylinositol 3-kinase activity in human airway smooth muscle cells

Discussion

Member takes

Implication

Key Points

Cite This Study

TGF-β1 modulates EGF-stimulated phosphatidylinositol 3-kinase activity in human airway smooth muscle cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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