Why the study?
Viral noncoding RNAs play important roles in the viral life cycle, with many viruses employing different mechanisms to produce them.
The study identifies a novel subgenomic noncoding RNA produced by the avian infectious bronchitis virus via a discontinuous transcription mechanism.
Extends models of coronavirus transcription; leaves open functional roles in IBV pathogenesis.
Viral noncoding (nc) RNAs have been shown to play important roles in viral life cycle. Many viruses employ different mechanism to produce ncRNAs. Here, we report that coronavirus infectious bronchitis virus (IBV) produces a novel ncRNA in virus-infected cells. This ncRNA consists of 563 nucleotides excluding a poly(A) tail, is mainly derived from the 3'-untranslated region of IBV genome, and contains a 63-nt-long of terminal leader sequence derived from the 5' end of the viral genome. Using mutagenesis and reverse genetics, we reveal that this ncRNA is a subgenomic RNA generated by discontinuous transcription mechanism.
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An et al. (2019) studied this question.
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