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February 26, 2021Diabetes & Metabolism JournalOpen Access

G-CSF significantly reduced the apoptotic index in diabetic rats compared with saline (25.12%±4.24% vs. 34.51%±3.93%, P<0.05) and significantly increased Beclin-1 and the LC3-II/LC3-I ratio while decreasing P62.

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Why the study?

The mechanisms underlying the anti-apoptotic effects of G-CSF in diabetic cardiomyopathy were not fully understood.

Does granulocyte-colony stimulating factor (G-CSF) reduce cardiomyocyte apoptosis by up-regulating autophagy in a model of diabetic cardiomyopathy?

Population

Rats with induced diabetic cardiomyopathy and high-glucose cultured rat H9c2 cardiac cells

Comparison

G-CSF treatment vs control or autophagy inhibition

Design

In vivo and in vitro animal experimental study

Authors

GSGuang-Yin ShenJSJeong‐Hun ShinYSYi-Sun Song

Discussion

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Overview

Autophagy may mediate G-CSF anti-apoptotic effects in diabetic cardiomyopathy models; hypothesis-generating, clinical translation untested.

Structured PICO

Does granulocyte-colony stimulating factor (G-CSF) reduce cardiomyocyte apoptosis by up-regulating autophagy in a model of diabetic cardiomyopathy?

P
Population
Male Sprague-Dawley rats (aged 7 weeks, weighing 210-230 g) with diabetic cardiomyopathy induced by high-fat diet and low-dose streptozotocin (n=22), and rat H9c2 cardiac cells cultured under high glucose conditions.
I
Intervention
Recombinant human granulocyte-colony stimulating factor (G-CSF) 200 μg/kg/day injected intraperitoneally for 5 days (in vivo); 3 μg/mL G-CSF (in vitro).
C
Comparator
Intraperitoneal injection of an equivalent volume of saline for 5 days (in vivo); high glucose media without G-CSF (in vitro).
O
Outcome
Extent of cardiomyocyte apoptosis (measured by TUNEL assay and flow cytometry) and autophagy-related protein levels (Beclin-1, LC3-II/LC3-I ratio, and P62).surrogate

G-CSF attenuates cardiomyocyte apoptosis in diabetic cardiomyopathy by up-regulating autophagy, highlighting a potential mechanistic pathway for treating diabetic heart disease.

Cite This Study

Shen et al. (2021) studied this question.

synapsesocial.com/papers/6a87229048ef9afcd2a262c1https://doi.org/10.4093/dmj.2020.0049
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Granulocyte colony-stimulating factor reduces the endoplasmic reticulum stress in a rat model of diabetic cardiomyopathy2021 · 8 citations
  2. 2The Possible Alleviating Effect of Granulocyte-Colony Stimulating Factor (G-CSF) on High Fat Diet-Induced Cardiomyopathy in Albino Rat: Histological, Immunohistochemical and Ultrastructural Study2024
  3. 3Effects of granulocyte-colony stimulating factor (G-CSF) on diabetic cardiomyopathy in Otsuka Long-Evans Tokushima Fatty rats2011 · 27 citations
  4. 4The Antiapoptotic Effect of Granulocyte Colony-stimulating Factor Reduces Infarct Size and Prevents Heart Failure Development in Rats2011 · 17 citations
  5. 5CARDIOPROTECTIVE EFFECTS OF GRANULOCYTE COLONY‐STIMULATING FACTOR IN ANGIOTENSIN II‐INDUCED CARDIAC REMODELLING2008 · 11 citations