September 1, 1994Journal of VirologyOpen Access

Identification of terminal adenylyl transferase activity of the poliovirus polymerase 3Dpol

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Population

Purified poliovirus RNA-dependent RNA polymerase (3Dpol) expressed in Escherichia coli

Design

Preclinical

Authors

Discussion

Reveals novel terminal adenylyl transferase activity in poliovirus 3Dpol; leaves open its role in viral RNA replication.

Population

Purified poliovirus RNA-dependent RNA polymerase (3Dpol) expressed in Escherichia coli

Intervention

Terminal adenylyl transferase (TATase) activity assay using chemically synthesized 12-nt and 13-nt RNA oligomers

Outcome

Identification and characterization of TATase activity (addition of AMP to the 3' ends of RNA)surrogate

Poliovirus RNA-dependent RNA polymerase 3Dpol possesses a novel terminal adenylyl transferase activity that may function in viral RNA replication.

Neufeld et al. (1994) studied this question.

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