Changes of Calcium Phosphate Partition and Heat Stability During Manufacture of Sterilized Concentrated Milk

Changes of partition of calcium and inorganic phosphate during the manufacture of concentrated milk were examined. Because of the difficulty in separating concentrated milk into colloidal and dispersal phases, preliminary separation of some fat and protein was effected by ultracentrifugation. The resulting serum was separated into a colloidal-free phase by ultrafiltration, and volume correction factors were obtained by measurement of the completely soluble chloride ion in the initial milk and in its ultrafiltrate. During processing of milk, both calcium and inorganic phosphate are transferred progressively from the soluble to the colloidal phase, and during heat sterilization the process continues up to the clotting point.